L. Razeghi; M. Azizi; S.M. Ziaratnia; A.R. Bagheri; S.H. Nemati
Abstract
K. odoratissima Mozaff., belonging to the Apiaceae family, is a known medicinal and forage species, endemic to Iran. It has not been reported in other regions of the world. Due to the excessive harvesting in the early period of growth and the relatively high time required for the establishment and seed ...
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K. odoratissima Mozaff., belonging to the Apiaceae family, is a known medicinal and forage species, endemic to Iran. It has not been reported in other regions of the world. Due to the excessive harvesting in the early period of growth and the relatively high time required for the establishment and seed production, this species does not have the opportunity to revitalize and seed production and for this reason, it is considered as one of the endangered species of Iran. Advances in tissue culture can be most effective in propagation of endangered plants as well as increasing the genetic potential of medicinal plants. To study the effect hormonal treatments on callus induction sterile seedling explants were prepared. Treatments included MS basal medium along with different hormonal levels (2,4-D (0.5, 1 and 2 mg/l) or a1, a2, a3 + Kin (0, 0.5 and 1 mg/l) or b1, b2, b3) and Ms basal medium with (NAA (0.5, 1 and 2 mg/l) or a4, a5, a6 + BA (0, 0.5 and 1 mg/l) or b4, b5, b6). After a month, growth (size), weight and callus induction percentage were measured and compared. Considering the above traits, the maximum size of callus (6.41mm) and the most callus induction percentage (93%) were obtained at 2,4-D (2mgl-1)+kin (0.5mgl-1) while the highest callus weight was obtained at NAA (1mgl-1) + BA (0.5mgl-1).Combined hormones (2mg/l 2,4-D + 0.5 mg/l kin) and (2mg/l NAA + 0.5mg/l BA) in two media, MS and B5, along with three antioxidants (PVP, PVPP and PVP + PVPP), totally 12 treatments, were evaluated within four consecutive weeks under broth condition and cell suspension establishment. According to the results, significant differences were found (p< 0.05) among the factors affecting cell dry weight while these factors had no significant effect on fresh weight. The highest fresh and dry weights were obtained at B5 medium with PVP (0.1) (1.6940 gr). Fresh and dry weight of cells showed an increasing trend during the consecutive weeks.
F. Oroojalian; R. Kasra-Kermanshahi; M. Azizi; M.R. Bassami
Abstract
Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial ...
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Essential oils of three medicinal plants species, including Carum copticum (L.) C. B. Clarke, Bunium persicum (Boiss.) B. Fedtsch. and Cuminum cyminum L., were obtained by hydrodistillation and their constituents were analyzed by GC and GC/MS using retention indices and fragmentation patterns. The antibacterial effects (MIC and MBC) of the essential oils were assessed on several food-borne pathogens, namely Staphylococcus aureus, Bacillus cereus, Escherichia coli O157: H7, Salmonella enteritidis and Listeria monocytogenes by microdilution technique using ELISA reader. Because of the combinatory usage of Bunium persicum and Cuminum cyminum in folk medicine, the affect of essential oil on food organoleptic properties and MIC values of the plants, interaction of Bunium persicum and Cuminum cyminum essential oils were also studied by FICindex determination using modified dilution checkboard method. The results showed that there are noticeable differences between the essential oils as their constituents as concerned, while ρ-cymen and γ-terpinene detected in all essential oils in different percentage. The main components of essential oils of C. copticum were thymol (48.4%), ρ-cymene(21.8%) and γ-terpinene (21.3%). The major constituents of B. persicum were γ-terpinene (44.2%), cuminaldehyde (16.9%), γ-terpinen-7-al (10.5%), and ρ-cymen (8%) while those of C. cyminum were cuminaldehyde (30.2%), ρ-cymene(14.1%), γ-terpinene (12.8%), and safranal (9.4%). The ranges of minimum inhibitory concentrationof the oils were 0.03-0.5,0.18-3.0, and 0.37-3.0 mg/ml, respectively, for C. copticum, B. persicum and C. cyminum. Moreover, the combination of B. persicum and C. cyminum essential oils confirmed synergistic and additive activities against the pathogens. In conclusion, although the MIC of Bunium persicum and Cuminum cyminum essential oils were lower than C. copticum, but combinatory usage of these essential oils especially against gram-positive bacteria produced promissing results. So application of these essential oils is recommended in combination as an efficients and complementary method for control of food borne pathogens with lower side effects on organolpetic properties of food.
M.T. Ebadi; M. Azizi; R. Omidbaigi; M. Hassanzadeh khayyat
Abstract
In order to study the effect of sowing date and harvest frequency on flower yield, essential oil percent and composition of chamomile (Matricaria recutita L.) CV. Presov, prepared from Slovakia, an experiment was conducted. The experiment was split-plot method based on randomized complete block design ...
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In order to study the effect of sowing date and harvest frequency on flower yield, essential oil percent and composition of chamomile (Matricaria recutita L.) CV. Presov, prepared from Slovakia, an experiment was conducted. The experiment was split-plot method based on randomized complete block design (RCBD) with three replications. Main plots consisted of three sowing dates (6th Nov, 5th Mar, and 4th Apr) and sub-plots included three harvest frequencies (first, second and third). Evaluated traits were dry flower yield, essential oil percentage and yield, yield of b-farnesene, a-bisabolol oxide B, a-bisabolol, chamazulene, a-bisabolol oxide A. The results showed that sowing date, harvest frequency and their interaction had significant effect on these parameters. Based on the results, the most dry flower yield (40 g/m2) was obtained from the second harvest of 6th November. Also the highest essential oil content (0.72 percent w/w), essential oil yield (0.26 g/m2) and a-bisabolol yield (0.2375 g/m2) were obtained from the second harvest of March and the most chamazulene yield (0.0473 g/m2) was obtained from the third harvest of March that it had a little difference with second harvest. According to the results, the best chamomile quality was attained in second harvest of March sowing date in Mashhad condition.
M.T. Ebadi; M. Azizi; R. Omidbaigi; M. Hassanzadeh Khayyat
Abstract
In this research, the effect of sowing date and seeding level on flower yield, essential oil and chamazulene content of chamomile (Matricaria recutita L.) CV. Presov, purchased from Slovakia, was studied. The experimental was split-plot in the basic of randomized complete blocked design (RCBD) with three ...
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In this research, the effect of sowing date and seeding level on flower yield, essential oil and chamazulene content of chamomile (Matricaria recutita L.) CV. Presov, purchased from Slovakia, was studied. The experimental was split-plot in the basic of randomized complete blocked design (RCBD) with three replications. Main plots consisted of three sowing dates (6 Nov., 5 Mar. and 4 Apr.) and sub-plots included three seeding levels (0.2, 0.4 and 0.8 g/m2). Evaluated traits were plant height, number of plants in plot, diameter of flower, fresh and dry flower yield, essential oil yield, essential oil and chamazulene percent. The results showed that sowing date had significant effect but seeding levels had only significant effect on number of plants in plot and essential oil yield. There are significant effects between sowing date and seeding level as measured traits are concerned. Based on the results, highest plant (47.4 cm), the most number of plants in plot (135.4 plants), the most fresh and dry flower yield (749.1 and 175.1 g/m2) were obtained from the plants which sown on 6 of Nov. but highest percentage of essential oil and chamazulene (0.59 and 5.62 percent respectively) and essential oil yield (0.79 g/m2) was obtained from the plants which sown on 5 of Mar. According to the results of their interaction, highest plant (49.7 cm), the most yield of wet and dry flower yield (810 and 198.2 g/m2) were obtained from the plots which sown on 6 of Nov. with 0.8 g/m2 but the most essential oil and chamazulene content (0.63 and 5.9 w/w percent respectively) and essential oil yield (0.97 g/m2) was obtained from the plots that were sown on 5 of Mar. with 0.4 g/m2. According to the results, the most suitable sowing date and seeding level in Mashhad condition is 5th Mar. with 0.4 g/m2 seeds.
M.Sh. Daneshmandi; M. Azizi
Abstract
In this research, the inhibitory effect of Eucalyptus globulus Labill. on germination and rhizome growth of Cynodon dactylon (L.) Pers. weed in three separate experiments were studied. In first experiment, after growing the weed, effects of different concentration of essential oils (20ppm, 50ppm, 100ppm) ...
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In this research, the inhibitory effect of Eucalyptus globulus Labill. on germination and rhizome growth of Cynodon dactylon (L.) Pers. weed in three separate experiments were studied. In first experiment, after growing the weed, effects of different concentration of essential oils (20ppm, 50ppm, 100ppm) as spray treatment on growth of the weed were investigated. In second experiment, effect of different level of Eucalyptus leaf litter (2, 5 and 10 w/w) as mulch treatments on germination of weed rhizome growth in a pot experiment were studied. In third experiment, effect of different concentrations of essential oils (20ppm, 50ppm, 100ppm) and leaf extract (25%, 50% and 75% water alcoholic extract) on in vitro rhizome germination were studied. Results indicated that leaf mulch had strong inhibitory effects on rhizome germination, that rhizome germination completely stopped in 50% w/w of mulch treatments. The highest number of germinated node (10.75 node/rhizome) was obtained in control treatment and the lowest one (1.5 node/rhizome) was detected in 10% mulch treatment. Essential oils spraying also showed inhibitory effect. After four months of spraying, all weeds became dried and died. Extract treatments also reduced growth of the weed as in lower concentration of extract (25%), germination of the rhizome was almost 66%, but in higher concentration (50% and 75%) complete inhibition of germination process take place. It was concluded that Eucalyptus globulus has a good potential to control Cynodon dactylon weed and it can be used as a herbicide in organic culture of the agricultural crops.
A. Ghani; M. Azizi; A. Tehranifar
Abstract
Achillea (Yarrow) is one of the most important genera of the Asteraceae family and different species grow wild in different parts of Iran. These species have ornamental and medicinal potential for use in drought area. The aim of this study was evaluating the effect of drought stress induce by Poly Ethylene ...
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Achillea (Yarrow) is one of the most important genera of the Asteraceae family and different species grow wild in different parts of Iran. These species have ornamental and medicinal potential for use in drought area. The aim of this study was evaluating the effect of drought stress induce by Poly Ethylene Glycol 6000 (PEG) on germination percentage and rate of Achillea species. An experiment in randomized complete block design (RCBD) with two factors in three replications was conducted. The first factor included 5 drought level (0, -5, -7, -9 and -20 bar that zero bar as control) and second factor included 6 species of Achillea (A. eriophora, A. millefolium, A. filipendula, A. nobilis, A. wilhelmsii and A. biebersteinii). The seed were collected from Shiraz, Mashhad and Karaj area. According to the results of statistical analysis, different levels of drought stress and type of species had significant effect (p<0.01) on measured characteristics (germination percentage, germination rate and radicle length). In this species radicle length affected by drought stress less than other traits and germination percentage was the best trait for drought stress evaluation. Finally, A. millefolium and A. eriophora have the highest drought tolerance and the lowest drought tolerance belongs to A. biebesteinii and A. nobilis. The A. wilhelmsii and A. filipendula have relative drought tolerance in germination stage.
